fa اثرات افزودن مکمل آنزیمی تریپسین خوکی به جیره کپور معمولی (Cyprinus carpio) بر رشد، ترکیبات لاشه، شاخص‌های بیوشیمیایی خون و فعالیت آنزیمی روده تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif">&nbsp;&nbsp;&nbsp;&nbsp; <span b="" lang="FA" style="font-family:" zar=""><span style="color:black">این مطالعه باهدف بررسی اثربخشی مکمل آنزیمی تریپسین خوکی در جیره غذایی ماهی کپور معمولی جهت بهبود رشد ماهی و ارتقا عملکرد دستگاه گوارش انجام شد</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span><b> </b><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">تعداد 120 قطعه ماهی کپور معمولی (</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Cyprinus carpio</span></span></span></i><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">) </span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">با میانگین وزن (12/0</span></span><span lang="FA" style="font-size:9.0pt"><span style="color:black">&plusmn;</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">45/15 گرم)</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black"> به‌طور تصادفی به چهار گروه تقسیم شدند. ماهیان با جیره‌های غذایی حاوی سطوح مختلف تریپسین (</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">01/0، 02/0 و 04/0 درصد</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">) به مدت هشت هفته تغذیه شدند و پارامترهای مختلف رشد، شاخص‌های بیوشیمیایی خون و فعالیت آنزیمی روده در آن‌ها اندازه‌گیری شدند</span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span><b> </b><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">نتایج نشان داد که استفاده از تریپسین در جیره به‌طور قابل‌توجهی عملکرد رشد را بهبود می‌بخشد و به افزایش وزن بدن و بهبود ضریب تبدیل غذایی منجر می‌شود </span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">(</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><0/05</span></span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">)</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">. غلظت بهینه تریپسین 02/0 درصد تعیین شد. درحالی‌که مکمل‌سازی جیره با آنزیم تأثیر قابل‌توجهی بر ترکیب شیمیایی بدن ماهی نداشت </span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">(</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">>0/05</span></span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">)</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">، منجر به افزایش جزئی فعالیت تریپسین در روده شد </span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">(</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">p>0/05</span></span></span></i><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">)</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">. پارامترهای بیوشیمیایی خون مانند گلوکز، پروتئین کل، آلبومین، گلوبولین و نیتروژن اوره&shy;ای خون در تیمارهای مختلف اختلاف معنی‌داری با یکدیگر نداشتند.<b> </b>یافته‌های این مطالعه نشان داد که مکمل‌سازی جیره غذایی با تریپسین می‌تواند به‌طور مؤثری عملکرد رشد ماهی کپور معمولی را بهبود بخشد </span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">(</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><0/05</span></span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black">)</span></span><span b="" lang="FA" style="font-family:" zar=""><span style="color:black"> و به‌عنوان یک استراتژی پایدار برای بهینه‌سازی تولید آبزیان و کاهش هزینه‌های خوراک برجسته شود. </span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span><br> <br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Introduction</span></span></b><b><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black"> The global surge in population has intensified the demand for protein sources, particularly from aquaculture. While fish farming has seen significant growth, challenges such as resource constraints and escalating production costs persist. </span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">This study was designed to evaluate the efficacy of dietary supplementation with porcine trypsin enzyme in enhancing growth performance and improving digestive system functionality in common carp (<i>Cyprinus carpio</i></span></span><span b="" dir="RTL" lang="AR-SA" style="font-family:" zar=""><span style="color:black">(</span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials and Methods:</span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black"> A total of 120 common carp,</span></span><span new="" roman="" style="font-family:" times=""><span style="color:black"> averaging 15.45&plusmn;0.12 g in initial body</span></span> <span new="" roman="" style="font-family:" times=""><span style="color:black">weight,</span></span><span new="" roman="" style="font-family:" times=""><span style="color:black"> were randomly </span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">allocated </span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">into four </span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">experimental </span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">groups.</span></span><span new="" roman="" style="font-family:" times=""><span style="color:black"> The fish were fed dietary regimens containing varying levels of trypsin (0.01%, 0.02%, and 0.04%) over a period of eight weeks. Subsequently, various growth parameters, blood biochemical indices, and intestinal enzyme activity were measured.</span></span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Results</span></span></b><b><span new="" roman="" style="font-family:" times=""><span style="color:black"> and Discussion</span></span></b><b><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></b> <span new="" roman="" style="font-family:" times=""><span style="color:black">The results demonstrated that the inclusion of trypsin in the diet significantly enhanced growth performance, leading to increased body weight and improved feed conversion ratio (<i>p</i><0/05). The optimal concentration of trypsin was determined to be 0.02%. While dietary supplementation with the enzyme did not significantly affect the proximate composition of the fish (<i>p</i>>0/05), it led to a slight increase in trypsin activity in the intestine (<i>p</i>>0/05). Finally, the blood biochemical parameters, including glucose, total protein, albumin, globulin, and blood urea nitrogen, did not exhibit significant differences among the various treatment groups (<i>p</i>>0/05).</span></span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b> <span new="" roman="" style="font-family:" times="">The findings of this study demonstrated that dietary supplementation with trypsin can effectively enhance the growth performance of common carp. Furthermore, it may serve as a sustainable strategy for optimizing aquaculture production and reducing feed costs. </span></span></span></span></div> <span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span> رشد, ضریب تبدیل, تریپسین, پروتئین, هضم, دستگاه گوارش Growth, Feed Conversion Ratio, Trypsin, Protein, Digestion, Gastrointestinal System 1 19 http://aqudev.lahijan.iau.ir/browse.php?a_code=A-10-647-1&slc_lang=fa&sid=1 Ali Khosravanizadeh علی خسروانی زاده akhosravanizadeh@gmail.com 100319475328460011728 100319475328460011728 Yes Department of Aquatic sciences, Hamoun International Wetland Institute, Research Institute of Zabol, Zabol, Iran گروه علوم آبزیان، پژوهشکده تالاب بین‌‌المللی هامون، پژوهشگاه زابل، زابل، ایران Abdolali Rahdari عبدالعلی راهداری 100319475328460011729 100319475328460011729 No Hashem Khandan Barani هاشم خندان بارانی 100319475328460011730 100319475328460011730 No