fa بررسی اثر ضد‌‌میکروبی‌کلوئید نانوذرات نقره علیه باکتری‌های .Bacillus spp و Vibrio harveyi و سینتیک رشد آنها تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span b="" lang="FA" style="font-family:" zar="">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; شیوع عفونت</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">های</span> <span b="" lang="FA" style="font-family:" zar="">میکروبی</span> <span b="" lang="FA" style="font-family:" zar="">در آبزیان از</span> <span b="" lang="FA" style="font-family:" zar="">چالش</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">های</span> <span b="" lang="FA" style="font-family:" zar="">مهم</span> <span b="" lang="FA" style="font-family:" zar="">بهداشتی</span> <span b="" lang="FA" style="font-family:" zar="">محسوب</span> <span b="" lang="FA" style="font-family:" zar="">می</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">شود</span> <span b="" lang="FA" style="font-family:" zar="">و آبزی‌پروران</span> <span b="" lang="FA" style="font-family:" zar="">در</span> <span b="" lang="FA" style="font-family:" zar="">این</span> <span b="" lang="FA" style="font-family:" zar="">زمینه</span> <span b="" lang="FA" style="font-family:" zar="">با مشکلات</span> <span b="" lang="FA" style="font-family:" zar="">عدیده&shy;ای</span> <span b="" lang="FA" style="font-family:" zar="">بویژه</span> <span b="" lang="FA" style="font-family:" zar="">مقاومت</span> <span b="" lang="FA" style="font-family:" zar="">باکتری</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">ها</span> <span b="" lang="FA" style="font-family:" zar="">نسبت</span> <span b="" lang="FA" style="font-family:" zar="">به</span> <span b="" lang="FA" style="font-family:" zar="">پادزیست‌ها</span> <span b="" lang="FA" style="font-family:" zar="">مواجه&shy;هستند. هدف</span> <span b="" lang="FA" style="font-family:" zar="">این</span> <span b="" lang="FA" style="font-family:" zar="">پژوهش،</span> <span b="" lang="FA" style="font-family:" zar="">ارزیابی</span> <span b="" lang="FA" style="font-family:" zar="">مقایسه</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">ای</span> <span b="" lang="FA" style="font-family:" zar="">فعالیت</span> <span b="" lang="FA" style="font-family:" zar="">ضد&shy;باکتریایی</span> <span b="" lang="FA" style="font-family:" zar="">نانوذرات نقره</span> <span b="" lang="FA" style="font-family:" zar="">علیه</span> <span b="" lang="FA" style="font-family:" zar="">دو نمونه باکتری گرم مثبت (.</span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bacillus spp</span></span></i><span b="" lang="FA" style="font-family:" zar="">) و گرم منفی</span> <span b="" lang="FA" style="font-family:" zar="">(</span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Vibrio harveyi</span></span></i><span b="" lang="FA" style="font-family:" zar="">)</span> <span b="" lang="FA" style="font-family:" zar="">بود.</span> <span b="" lang="FA" style="font-family:" zar="">در این</span> <span b="" lang="FA" style="font-family:" zar="">مطالعه تجربی-آزمایشگاهی برای بررسی فعالیت</span> <span b="" lang="FA" style="font-family:" zar="">ضد&shy;باکتریایی</span> <span b="" lang="FA" style="font-family:" zar="">کلوئید نانوذرات نقره</span> <span b="" lang="FA" style="font-family:" zar="">از</span> <span b="" lang="FA" style="font-family:" zar="">روش انتشار</span> <span b="" lang="FA" style="font-family:" zar="">چاهک</span> <span b="" lang="FA" style="font-family:" zar="">با</span> <span b="" lang="FA" style="font-family:" zar="">استفاده</span> <span b="" lang="FA" style="font-family:" zar="">از</span> <span b="" lang="FA" style="font-family:" zar="">اندازه‌گیری</span> <span b="" lang="FA" style="font-family:" zar="">قطر</span> <span b="" lang="FA" style="font-family:" zar="">هاله</span> <span b="" lang="FA" style="font-family:" zar="">مهاری،</span> <span b="" lang="FA" style="font-family:" zar="">تعیین</span> <span b="" lang="FA" style="font-family:" zar="">حداقل</span> <span b="" lang="FA" style="font-family:" zar="">غلظت</span> <span b="" lang="FA" style="font-family:" zar="">بازدارندگی</span> <span b="" lang="FA" style="font-family:" zar="">و</span> <span b="" lang="FA" style="font-family:" zar="">کشندگی توسط روش</span> <span b="" lang="FA" style="font-family:" zar="">رقیق&shy;سازی</span> <span b="" lang="FA" style="font-family:" zar="">در</span> <span b="" lang="FA" style="font-family:" zar="">لوله و سنجش سینتیک رشد بر</span> <span b="" lang="FA" style="font-family:" zar="">روی این</span> <span b="" lang="FA" style="font-family:" zar="">دو</span> <span b="" lang="FA" style="font-family:" zar="">گونه</span> <span b="" lang="FA" style="font-family:" zar="">باکتریایی</span> <span b="" lang="FA" style="font-family:" zar="">استفاده</span> <span b="" lang="FA" style="font-family:" zar="">شد.</span> <span b="" lang="FA" style="font-family:" zar="">بر</span> <span b="" lang="FA" style="font-family:" zar="">اساس</span> <span b="" lang="FA" style="font-family:" zar="">تست</span> <span b="" lang="FA" style="font-family:" zar="">انتشار</span> <span b="" lang="FA" style="font-family:" zar="">چاهک</span> <span b="" lang="FA" style="font-family:" zar="">در</span> <span b="" lang="FA" style="font-family:" zar="">آگار،</span> <span b="" lang="FA" style="font-family:" zar="">بیشترین</span> <span b="" lang="FA" style="font-family:" zar="">میزان</span> <span b="" lang="FA" style="font-family:" zar="">هاله</span> <span b="" lang="FA" style="font-family:" zar="">عدم</span> <span b="" lang="FA" style="font-family:" zar="">رشد</span> <span b="" lang="FA" style="font-family:" zar="">مربوط</span> <span b="" lang="FA" style="font-family:" zar="">به باکتری</span> <span b="" lang="FA" style="font-family:" zar="">باسیلوس</span> <span b="" lang="FA" style="font-family:" zar="">با</span> <span b="" lang="FA" style="font-family:" zar="">قطر</span> <span b="" lang="FA" style="font-family:" zar="">15/0 </span><span lang="FA" new="" roman="" style="font-family:" times="">&plusmn;</span><span b="" lang="FA" style="font-family:" zar="">6/44 میلی</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span b="" lang="FA" style="font-family:" zar="">متر</span> <span b="" lang="FA" style="font-family:" zar="">مشاهده</span> <span b="" lang="FA" style="font-family:" zar="">شد. حداقل</span> <span b="" lang="FA" style="font-family:" zar="">غلظت</span> <span b="" lang="FA" style="font-family:" zar="">مهار</span> <span b="" lang="FA" style="font-family:" zar="">کنندگی در دو باکتری </span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bacillus spp.</span></span></i><span b="" lang="FA" style="font-family:" zar=""> و&nbsp; </span>&nbsp;<i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">V. harveyi</span></span></i><span b="" lang="FA" style="font-family:" zar=""> به ترتیب 95/1 و 91/3 و</span> <span b="" lang="FA" style="font-family:" zar="">حداقل</span> <span b="" lang="FA" style="font-family:" zar="">غلظت</span> <span b="" lang="FA" style="font-family:" zar="">کشندگی نیز 91/3 میلی&shy;</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span b="" lang="FA" style="font-family:" zar="">گرم در میلی</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span b="" lang="FA" style="font-family:" zar="">لیتر</span> <span b="" lang="FA" style="font-family:" zar="">بود</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span b="" lang="FA" style="font-family:" zar=""> بررسی سینتیک رشد نیز تائیدی بر نتایج به دست آمده بود، &nbsp;به</span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&shy;</span></span><span b="" lang="FA" style="font-family:" zar="">طوری که رشد باکتری&shy;های </span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Bacillus spp</span></span></i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span b="" lang="FA" style="font-family:" zar=""> 5 ساعت و </span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;V. harveyi</span></span></i><span b="" lang="FA" style="font-family:" zar="">6 ساعت بعد از مواجهه با نانوذرات نقره متوقف شد. این مطالعه نشان داد که کلوئید نانوذرات نقره اثرات</span> <span b="" lang="FA" style="font-family:" zar="">ضدباکتریایی بیشتری</span> <span b="" lang="FA" style="font-family:" zar="">بر</span> <span b="" lang="FA" style="font-family:" zar="">باکتری</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">های</span> <span b="" lang="FA" style="font-family:" zar="">گرم</span> <span b="" lang="FA" style="font-family:" zar="">مثبت</span> <span b="" lang="FA" style="font-family:" zar="">در</span> <span b="" lang="FA" style="font-family:" zar="">مقایسه</span> <span b="" lang="FA" style="font-family:" zar="">با</span> <span b="" lang="FA" style="font-family:" zar="">باکتری</span><span b="" lang="AR-SA" style="font-family:" zar="">‌</span><span b="" lang="FA" style="font-family:" zar="">های</span> <span b="" lang="FA" style="font-family:" zar="">گرم</span> <span b="" lang="FA" style="font-family:" zar="">منفی دارد.و البته می‌تواند به&shy;عنوان گزینه مناسبی برای حذف هر دو نوع باکتری گرم مثبت و منفی، بویژه حذف باکتری‌های عفونت‌زای آبزیان استفاده &shy;گردد.</span></span></span></span></span></span></span></span></span> Microbial infections outbreak in aquatics are important challenges, and farmers have major difficulties dealing with microbes especially regarding their antibiotic resistance. The purpose of this study was comparative evaluating of antimicrobial activity of silver nanoparticles (Ag NPs) against one gram-positive bacteria (<em>Bacillus spp</em>) and a gram-negative (<em>Vibrio harveyi</em>). In this experimental study, the antibacterial effects of Ag NPs was determined by agar well diffusion method through the measurement of the inhibitory zone diameter and micro-broth dilution for determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the results of well diffusion test in agar, the maximum diameter of inhibition zone (44.6 &plusmn; 0.15 mm) was related to <em>Bacillus spp</em>. MIC of Ag NPs against <em>Bacillus spp</em> and <em>Vibrio harveyi </em>&nbsp;were 1.95 and 3.91 mg/ml respectively and MBC was recorded 3.91 mg/ml for both bacteria. Also the kinetic study confirmed the results, So that growth of Bacillus spp 5 hours and V. harveyi 6 hours after exposure to silver nanoparticles was stopped.This study showed that colloidal Ag NPs have higher bactericidal effects on gram-positive bacteria compared with gram-negative bacteria and of course can be a candidate for the elimination of both gram negative and gram positive bacteria, especially in control of infective aquatic microorganisms.<br> &nbsp; آبزی‌پروری, حداقل غلظت بازدارندگی, حداقل غلظت کشندگی, ضد‌باکتریایی, هاله عدم رشد. Antibacterial, Aquaculture, Minimum Bactericidal Concentration, Minimum Inhibitory Concentration, Zone of inhibition. 133 143 http://aqudev.lahijan.iau.ir/browse.php?a_code=A-10-487-1&slc_lang=fa&sid=1 Mahmoud Nafisi Bahabadi1 محمود نفیسی بهابادی nafisi2002@gmail.com 10031947532846008615 10031947532846008615 No Persian Gulf University of Bushehr دانشگاه خلیج فارس بوشهر فاطمه حسین پور دلاور fhosseinpour18@gmail.com 10031947532846008616 10031947532846008616 Yes دانشگاه علوم کشاورزی و منابع طبیعی گرگان مریم میربخش maryam.mirbakhsh@gmail.com 10031947532846008617 10031947532846008617 No پژوهشکده میگوی کشور خدابخش نیکنام niknam@pgu.ac.ir 10031947532846008618 10031947532846008618 No دانشگاه خلیج فارس بوشهر سید علی جوهری sajohari@gmail.com 10031947532846008619 10031947532846008619 No دانشگاه کردستان