The sturgeon farming industry is associated with the production of a significant volume of organic waste, including viscera, skin, and inedible tissues, the improper disposal of which causes environmental problems. In the present study, the possibility of using indigenous proteolytic bacteria to biodegrade these wastes and produce amino acid fertilizer was investigated. For this purpose, sturgeon wastes were converted into biosilage using proteolytic bacteria under a controlled process. And strains were isolated and screened from the fermentation medium. Molecular identification of the dominant strain was performed using 16S rRNA gene sequencing and was identified as a species of the genus Bacillus. The results showed that for every 5 kg of initial wastes, one kg of dry biosilage was produced.

 Although the total protein content of biosilage was lower than some chemical fertilizers, protein digestibility was significantly increased. The amino acid profile showed a predominance of alanine and phenylalanine in the final product. Chemical spoilage indicators including TVB-N, peroxide value and TBA were within acceptable limits, and the results of microbial analysis confirmed the absence of Salmonella and low levels of coliforms. Overall, the biosilage produced in this study has the potential to be used as a sustainable bio-amino acid fertilizer and a substitute for some of the conventional chemical fertilizers in terms of chemical composition, stability, microbial safety and amino acid richness.